Simplified immunoassay for rapid Dengue serotype diagnosis, revealing insensitivity to non-specific binding interference

Full serotype- and group-specific NS1 capture enzyme-linked immunosorbent assay for rapid differential diagnosis of dengue virus infection.

Dengue virus (DENV), a member of the Flavivirus family, has four distinct serotypes (DENV serotype 1 [DENV1], DENV2, DENV3, and DENV4) that require differentiation for the effective prevention of morbid disease. Early and rapid differentiation between flaviviruses remains challenging. Full assays combining four individual, serotype-specific and one group-specific nonstructural protein 1 (NS1) a...

Rapid dengue diagnosis and interpretation.

Outbreaks of dengue fever (DF) and dengue haemorrhagic fever (DHF) pose a serious threat to more than 85 tropical and sub-tropical countries throughout the world. The World Health Organization has identified dengue to be one of the most under-reported diseases, with actual cases in the millions. It is also recognised as one of the most important and rapidly rising mosquito-transmitted infection...

Serotype 1-specific monoclonal antibody-based antigen capture immunoassay for detection of circulating nonstructural protein NS1: Implications for early diagnosis and serotyping of dengue virus infections.

Rapid diagnosis and serotyping of dengue virus (DV) infections are important for timely clinical management and epidemiological control in areas where multiple flaviviruses are endemic. However, the speed and accuracy of diagnosis must be balanced against test cost and availability, especially in developing countries. We developed a specific antigen capture enzyme-linked immunosorbent assay (EL...

Virus Infections Early Diagnosis and Serotyping of Dengue Nonstructural Protein NS1: Implications for Immunoassay for Detection of Circulating Antibody-Based Antigen Capture Serotype 1-Specific Monoclonal

Pediatric Measles Vaccine Expressing a Dengue Antigen Induces Durable Serotype-specific Neutralizing Antibodies to Dengue Virus

Dengue disease is an increasing global health problem that threatens one-third of the world's population. Despite decades of efforts, no licensed vaccine against dengue is available. With the aim to develop an affordable vaccine that could be used in young populations living in tropical areas, we evaluated a new strategy based on the expression of a minimal dengue antigen by a vector derived fr...